P-4: Ethyl Pyruvate Against MTX- Induced Sperm Toxicity in Mice

Background: Methotrexate, an immunosuppressive drug, is a folic acid antagonist. This inhibits synthesis of thymidylate, serine, and methionine, which disrupts synthesis of DNA, RNA, and protein and leads to cell death. Ethyl pyruvate, a commercial food additive, is a lipophilic ester derivative of pyruvate .In previous reports, that has been shown to ameliorate organ dysfunction in a variety of in vivo models.Our purpose is to evaluate the effect of ethyl pyruvate on sperm count in MTX treated mice. Materials and Methods: 32 adult male mice weighing 26